Composite

Part:BBa_K2481114:Design

Designed by: Erin Kelly   Group: iGEM17_Lethbridge_HS   (2017-11-01)


Expression construct for Anthocyanin synthase (ANS) from Malus domestica


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 106


Design Notes

The ANS CDS sequence was codon optimized for expression in E. coli and included with a promoter, RBS and terminator that will function in E. coli.


Source

DNA sequence of the ANS CDS is based on the CDS sequence from Malus domestica identified in Uniprot.

References